Dr Anthony Duff
Staff Profile
Role at ANSTO
Anthony Duff is a structural biologist interested in the structure and function of biological macromolecules. Ultimately, the goal is to understand the mechanism of life at the molecular level. The field of structural biology is inherently an interdisciplinary and collaborative science involving a range of complementary methods.
Prominent examples are X-ray crystallography and nuclear magnetic resonance spectroscopy, which provide precise information on rigid biomolecular structures, and electron microscopy which provides moderate resolution structural information on large assemblies, including membrane structures. To complement these structural techniques, we aim to use small-angle X-ray and neutron scattering to characterise the dynamic structures of biological macromolecular assemblies.
Qualifications & Achievements
- Ph.D. from the School of Physics, The University of New South Wales, submitted 1999
Title: Crystallographic Studies of the Protein Rubisco: New Perspectives on Closure and Catalysis in this Two-State Metalloenzyme
Supervisor: Dr Paul M.G. Curmi, Protein Structure Group.
Co-supervisor: Prof. T. John Andrews, ANU
- B.Sc.(Hons) majoring in physics, from The University of New South Wales, 1993
- Graduate Certificate in Adult Education, from The University of New England, 2008
Publications
Enantiomer-specific binding of ruthenium(II) molecular wires by the amine oxidase of Arthrobacter globiformis Langley, DB; Brown, DE; Cheruzel, LE; Contakes, SM; Duff, AP; Hilmer, KM; Dooley, DM; Gray, HB; Guss, JM; Freeman, HC JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 130 (25):8069-8078 2008
Complexes of the copper-containing amine oxidase from Arthrobacter globiformis with the inhibitors benzylhydrazine and tranylcypromine Langley, DB; Trambaiolo, DM; Duff, AP; Dooley, DM; Freeman, HC; Guss, JM ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 64577-583 2008
The 1.23 angstrom structure of Pichia pastoris lysyl oxidase reveals a lysine-lysine cross-linkDuff, AP; Cohen, AE; Ellis, PJ; Hilmer, K; Langley, DB; Dooley, DM; Freeman, HC; Guss, JM ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 621073-1084 Part 9 2006
A C-terminal disulfide bond in the copper-containing amine oxidase from pea seedlings violates the twofold symmetry of the molecular dimer Duff, AP; Shepard, EM; Langley, DB; Dooley, DM; Freeman, HC; Guss, JM ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 621168-1173 Part 12 2006
The copper-containing amine oxidase from Arthrobacter globiformis: refinement at 1.55 and 2.20 angstrom resolution in two crystal forms Langley, DB; Duff, AP; Freeman, HC; Guss, JM ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 621052-1057 Part 11 2006
Reversible inhibition of copper amine oxidase activity by channel-blocking ruthenium(II) and rhenium(I) molecular wires Contakes, SM; Juda, GA; Langley, DB; Halpern-Manners, NW; Duff, AP; Dunn, AR; Gray, HB; Dooley, DM; Guss, JM; Freeman, HC PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 102 (38):13451-13456 2005
Crystal structures of recombinant human purple acid phosphatase with and without an inhibitory conformation of the repression loop Strater, N; Jasper, B; Scholte, M; Krebs, B; Duff, AP; Langley, DB; Han, RL; Averill, BA; Freeman, HC; Guss, JM JOURNAL OF MOLECULAR BIOLOGY 351 (1):233-246 2005
Using xenon as a probe for dioxygen-binding sites in copper amine oxidases Duff, AP; Trambaiolo, DM; Cohen, AE; Ellis, PJ; Juda, GA; Shepard, EM; Langley, DB; Dooley, DM; Freeman, HC; Guss, JM JOURNAL OF MOLECULAR BIOLOGY 344 (3):599-607 2004
Differential inhibition of six copper amine oxidases by a family of 4-(aryloxy)-2-butynamines: Evidence for a new mode of inactivation O'Connell, KM; Langley, DB; Shepard, EM; Duff, AP; Jeon, HB; Sun, G; Freeman, HC; Guss, JM; Sayre, LM; Dooley, DM BIOCHEMISTRY 43 (34):10965-10978 2004
The crystal structure of Pichia pastoris lysyl oxidase Duff, AP; Cohen, AE; Ellis, PJ; Kuchar, JA; Langley, DB; Shepard, EM; Dooley, DM; Freeman, HC; Guss, JM BIOCHEMISTRY 42 (51):15148-15157 2003
The transition between the open and closed states of rubisco is triggered by the inter-phosphate distance of the bound bisphosphate Duff, AP; Andrews, TJ; Curmi, PMG JOURNAL OF MOLECULAR BIOLOGY 298 (5):903-916 2000