|Deuterated fibronectin and Neutron Reflectivity |
Extracellular matrix (ECM) proteins such as fibronectin (FN), laminin and collagen can be used to create biomimetic surfaces for cell adhesion and tissue engineering. By immobilising biocompatible ECM proteins on a biomaterial surface the interaction between it and the host cells can be influenced.
Hydrogenous and deuterated (at NDF) variants of the fibronectin fragment, 9th-10th type III domains (FIII9-10) were investigated for their ability to mediate adhesion of cultured cells to TiO2 surface by neutron reflectivity on Platypus at the Australian Centre for Neutron Scattering.
McIntosh L, Whitelaw C, Rekas A, Holt SA, van der Walle CF. Interrogating protonated/deuterated fibronectin fragment layers adsorbed to titania by neutron reflectivity and their concomitant control over cell adhesion. Interface, 2015, 12(107). http://dx.doi.org/10.1098/rsif.2015.0164
|Storage in solid Metal-Organic Frameworks (MOF) |
Neutron diffraction experiments are used to unveil the site-specific binding properties of CO2 within MOF materials while systematically varying both the amount of CO2 and the temperature. Deuterated materials enabled a comprehensive study of carbon dioxide adsorption in the metal-organic frameworks M2 (dobdc) (M = Mg, Mn, Fe, Co, Ni, Cu, Zn).
|Deuterated biopolymer and Infrared (IR) Microspectroscopy |
Deuteration of bacterial biopolyesters to probe phase separation using the Infrared Microspectroscopy beamline at the Australian Synchrotron.
Russell RA, Darwish TA, Puskar L, Martin DE, Holden PJ, Foster LJR. Deuterated polymers for probing phase separation using infrared microspectroscopy. Biomacromolecules, 2014, 15(2): 644-649. http://dx.doi.org/10.1021/bm4017012
|Biomembrane research |
Dr. Anton Le Brun uses neutron scattering techniques in combination with deuteration to research phenomena that occur at biological membranes. This has included production of deuterated lipopolysaccharide (LPS) from E. coli for studying structural aspects of the Gram-negative bacterial outer membrane using neutron reflectometry.
Le Brun AP, Clifton LA, Halbert CE, Lin B, Meron M, Holden PJ, Lakey JH, Holt SA: Structural Characterisation of a Model Gram-negative Bacterial Surface using Lipopolysaccharides from Rough Strains of Escherichia coli. Biomacromolecules, 2013, 14(6):2014-2022. http://dx.doi.org/10.1021/bm400356m
|Structural characterisation of an HIV coat protein - calmodulin interation |
Structural characterisation of the interaction between:
Binding of HIV-1 MA protein to deuterated calmodulin, modelled from small-angle neutron scattering (SANS) conducted at multiple solvent contrasts.
|Triple-labelled hydrophobin (2H/15N/ 13C) and NMR |
Investigation of a fungal hydrophobin (EAS∆15) rodlet structure using recombinantly expressed 2H/15N/13C labelled EAS∆15 and solid-state NMR.
Morris VK, Linser R, Wilde KL, Duff AP, Sunde M, Kwan AH. Solid-state NMR spectroscopy of functional amyloid from a fungal hydrophobin: a well-ordered β–sheet core amidst structural heterogeneity. Angew Chem Int Ed, 2012, 51:12621 –12625. http://dx.doi.org/10.1002/anie.201205625
The morphology and structure of Organic Light Emitting Diodes (OLED)
Proposals for molecular deuteration should be submitted at http://neutron.ansto.gov.au. You can email email@example.com or contact Professor Peter Holden on + 61 (2) 9717 3991 or other NDF staff at any time.