Dr Anthony Duff
Role at ANSTO
Anthony Duff is a structural biologist interested in the structure and function of biological macromolecules. Ultimately, the goal is to understand the mechanism of life at the molecular level. The field of structural biology is inherently an interdisciplinary and collaborative science involving a range of complementary methods.
Prominent examples are X-ray crystallography and nuclear magnetic resonance spectroscopy, which provide precise information on rigid biomolecular structures, and electron microscopy which provides moderate resolution structural information on large assemblies, including membrane structures. To complement these structural techniques, we aim to use small-angle X-ray and neutron scattering to characterise the dynamic structures of biological macromolecular assemblies.
Qualifications & Achievements
- Ph.D. from the School of Physics, The University of New South Wales, submitted 1999
Title: Crystallographic Studies of the Protein Rubisco: New Perspectives on Closure and Catalysis in this Two-State Metalloenzyme
Supervisor: Dr Paul M.G. Curmi, Protein Structure Group.
Co-supervisor: Prof. T. John Andrews, ANU
- B.Sc.(Hons) majoring in physics, from The University of New South Wales, 1993
- Graduate Certificate in Adult Education, from The University of New England, 2008
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: An update
(2017) Acta Crystallographica Section D: Structural Biology, 73 (9), pp. 710-728.
Neutron scattering shows a droplet of oleic acid at the center of the BAMLET complex
(2017) Proteins: Structure, Function and Bioinformatics, 85 (7), pp. 1371-1378.
Golden, E., Yu, L.-J., Meilleur, F., Blakeley, M.P., Duff, A.P., Karton, A., Vrielink, A.
An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase
(2017) Scientific Reports, 7, art. no. 40517.
Phang, J.M., Harrop, S.J., Duff, A.P., Sokolova, A.V., Crossett, B., Walsh, J.C., Beckham, S.A., Nguyen, C.D., Davies, R.B., Glöckner, C., Bromley, E.H.C., Wilk, K.E., Curmi, P.M.G.
Structural characterization suggests models for monomeric and dimeric forms of full-length ezrin
(2016) Biochemical Journal, 473 (18), pp. 2763-2782.
Wood, K., Jeffries, C.M., Knott, R.B., Sokolova, A., Jacques, D.A., Duff, A.P.
Exploring the structure of biological macromolecules in solution using Quokka, the small angle neutron scattering instrument, at ANSTO
(2015) Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 798, art. no. 57835, pp. 44-51.
Duff, A.P., Wilde, K.L., Rekas, A., Lake, V., Holden, P.J.
Robust High-Yield Methodologies for 2H and 2H/15N/13C Labeling of Proteins for Structural Investigations Using Neutron Scattering and NMR
(2015) Methods in Enzymology, 565, pp. 3-25.
Rath, E.M., Duff, A.P., Håkansson, A.P., Knott, R.B., Church, W.B.
Small-angle X-ray scattering of BAMLET at pH 12: A complex of α-lactalbumin and oleic acid
(2014) Proteins: Structure, Function and Bioinformatics, 82 (7), pp. 1400-1408.
Duff, A.P., Trambaiolo, D.M., Cohen, A.E., Ellis, P.J., Juda, G.A., Shepard, E.M., Langley, D.B., Dooley, D.M., Freeman, H.C., Guss, J.M.
Using xenon as a probe for dioxygen-binding sites in copper amine oxidases
(2004) Journal of Molecular Biology, 344 (3), pp. 599-607.
Duff, A.P., Cohen, A.E., Ellis, P.J., Kuchar, J.A., Langley, D.B., Shepard, E.M., Dooley, D.M., Freeman, H.C., Guss, J.M.
The Crystal Structure of Pichia pastoris Lysyl Oxidase
(2003) Biochemistry, 42 (51), pp. 15148-15157.
Duff, A.P., Andrews, T.J., Curmi, P.M.G.
The transition between the open and closed states of rubisco is triggered by the inter-phosphate distance of the bound bisphosphate(2000) Journal of Molecular Biology, 298 (5), pp. 903-916.