Dr Stephen Holt

Instrument Scientist
Phone - +61 2  9717 3173


Stephen received his PhD (Physical Chemistry) from Griffith University in 1996. From there he moved into x-ray and then neutron scattering studies of surfaces and interface with Postdocs at the Australian Defence Force Acadamy and the Research School of Chemistry (Australian National University) in Canberra. In 1998 he was awarded an inaugural Australian Synchrotron Research Programme fellowship to study surfactant mediated crystallisation at air/liquid interfaces. Immediately prior to returning to Australia for a position at The Bragg Institute, ANSTO, he was an instrument scientist (neutron reflectometry) at the ISIS spallation neutron source in Oxfordshire, United Kingdom.

Stephens research interests are related to the development of interfacial structure in soft condensed matter systems. In recent years this has focussed particularly on the assembly of proteins and protein lipid mixtures at interfaces both for functional and conformation studies and in the development of realistic model membranes.


Selected publications

M Kreiner, C.R. Chillakuri, P. Pereira, M. Fairhead, Z. Li, H.J. Mardon, S.A. Holt, C.F. van der Walle. Orientation and surface coverage of adsorbed fibronectin cell binding domains and bound integrin alpha5beta1 receptors. Soft Matter, 2009, 5, 3954. DOI: 10.1039/b908706k.
S.A. Holt, A.P. Le Brun, C.F. Majkrzak, D.J. McGillivray, F. Heinrich, M. Lösche and J.H. Lakey. An ion channel containing model membrane: structural determination by magnetic contrast neutron reflectometry. Soft Matter, 5, 2576 2586, 2009. DOI: 10.1039/b822411k.
W.K. Fullagar, S.A. Holt and I.R. Gentle. Structure of SP-B/DPPC Mixed Films Studied by Neutron Reflectometry. Biophysical Journal, 95, 4829 4836, 2008.
A.P. Le Brun, S.A. Holt, D.S. Shah, C.F. Majkrzak and J.H. Lakey. Monitoring the assembly of antibody-binding membrane protein arrays using polarised neutron reflection. European Biophysics Journal, 37 (5), 639 645, 2008.
S.A. Holt, J.L. Ruggles, P.A. Reynolds and J.W. White. Structural Development of Silicated Films Self-Assembled at the Air/Water Interface. Physica B, 336 (1-2), 193-203, 2003.
J.L. Ruggles, E.P. Gilbert, S.A. Holt, P.A. Reynolds and J.W. White. Expanded Mesoporous Silicate Films Grown at the Air-Water Interface by the Addition of Hydrocarbons. Langmuir, 19, 793 800, 2003.
S.A. Holt, M.J. Henderson and J.W. White. Thermal Denaturation of Interfacial Protein Layers. Australian Journal of Chemistry, 55, 449 -459, 2002.
S.A. Holt, G.J. Foran and J.W. White. Observation of Hexagonal Crystalline Diffraction from Growing Silicate Films. Langmuir, 15(17), 2540 - 2542, 1999.
A.S. Brown, S.A. Holt, P.A. Reynolds, J. Penfold and J.W. White. Growth of Highly Ordered Thin Silicate Films at the Air - Water Interface. Langmuir, 14(19), 5532 - 5538, 1998.
A.S. Brown, S.A. Holt, M. Trau, Thien Dam and J.W. White. Mesoporous Silicate Film Growth at the Air - Water Interface - Direct Observation by X-ray Reflectivity. Langmuir, 13(24), 6363 - 6365, 1997.