Mr Nicholas Howell

Cell Biology Professional Officer
Phone - +61 2 9717 3071
   
Howell-Nick

Profile

Role at ANSTO

 
Nicholas works in the Bioanalytics group under Prof. Richard Banati providing expertise and support for the in vitro evaluation of novel compounds, peptides, proteins and particles. He also provides expertise and support for the analytical biology requirements of ANSTO LifeSciences and its collaborators in radiological and nuclear methodologies for applications.
 

Background

 
Graduating from Macquarie University with a BSc in Biotechnology, Nicholas has been working at ANSTO as a biologist for 5 years.
 


Areas of expertise and Research interests

 
Nicholas has expertise and interest in the application of radiological and nuclear techniques to the analysis of biological systems and structures. Other areas of expertise include in vitro pharmacology, RT-qPCR, immunohistochemistry, cell culture.
 

Selected publications

Callaghan, P. D., Wimberley, C. A., Rahardjo, G. L., Berghofer, P. J., Pham, T. Q., Jackson, T., Zahra, D., Bourdier, T., Wyatt, N., Greguric, I., Howell, N. R., Siegele, R., Pastuovic, Z., Mattner, F., Loc'h, C., Gregoire, M-C., Katsifis, A. Comparison of in vivo binding properties of the 18 kDa translocator protein (TSPO) ligands [18F]PBR102 and [18F]PBR111 in a model of excitotoxin-induced neuroinflammation. European Journal of Nuclear Medicine and Molecular Imaging, 42(1), 138-151 (2015). doi: 10.1007/s00259-014-2895-3


Banati, R. B., Middleton, R. J., Chan, R., Hatty, C. R., Kam, W. W-Y., Quin, C., Graeber, M. B., Parmar, A., Callaghan, P., Fok, S., Howell, N. R., Gregoire, M-C., Szabo, A. M., Pham, T., Davis, E., Liu, G-J. Positron emission tomography and functional characterization of a complete PBR/TSPO-knockout. Nature Communications, 5, 5452 (2014). doi: 10.1038/ncomms6452.


Krause-Heuer, A. M., Howell, N. R., Matesic, L., Dhand, G., Young, E. L., Burgess, L., Jiang, C. D., Lengkeek, N. A., Fookes, C. J. R., Pham, T. Q., Sobrio, F., Greguric, I., Fraser, B. H. A new class of fluorinated 5-pyrrolidinylsulfonyl isatin caspase inhibitors for PET imaging of apoptosis. MedChemComm, 2(4), 347-352 (2013). doi: 10.1039/C2MD20249B


Nguyen, V. H., Pham, T., Fookes, C., Berghofer, P., Greguric, I., Arthur, A., Mattner, F., Rahardjo, G., Davis, E., Howell, N., Gregoire, M-C., Katsifis, A., Shepherd, R. Synthesis and biological characterisation of 18F-SIG343 18F-SIG353, novel and high selectivity sigma2 radiotracers, for tumour imaging properties. EJNMMI Research, 3(1), 80 (2013). doi: 10.1186/2191-219X-3-80


Pellegrini, P. A., Howell, N. R., Shepherd, R. K., Lengkeek, N. A., Oehlke, E., Katsifis, A. G., Greguric, I. Synthesis and radiolabelling of DOTA-linked glutamine analogues with 67,68Ga as markers for increased glutamine metabolism in tumour cells. Molecules, 18(6), 7160-7178 (2013). doi: 10.3390/molecules18067160


Siegele, R., Howell, N., Callaghan, P. D., Pastuovic, Z. Investigation of elemental changes in brain tissues following excitotoxic injury. Nuclear Instruments and Methods in Physics Research Section B, 306, 125-128 (2013). doi: 10.1016/j.nimb.2012.12.050
 

Szabo, A. M., Howell, N. R., Pellegrini, P., Greguric, I., Katsifis, A.  Development and validation of competition binding assays for affinity to the extracellular matrix receptors, αvβ3 and αIIbβ3 integrin. Analytical Biochemistry, 423(1) 70-77. (2012)

 
Peyronneau, M., Mattner, F., Howell, N., Jiang, C., Pellegrini, P., Greguric, I., Loch, C., Katsifis, A. Metabolism of CLINDE, a peripheral benzodiazepine receptor SPECT ligand. European Journal of Nuclear Medicine and Molecular Imaging, 37(2), S242. (2010)
 
Mattner, F., Pham, T., Berghofer, P., Howell, N., Bourdier, T., Rahardjo, G., Kench, P. L., Zahra, D., Gregoire, M-C., Katsifis, A. Pharmacological evaluation of the Peripheral Benzodiazepine Receptor radioligand [123I]-CLINDE in a breast tumour model. 57th Annual Meeting of the Society of Nuclear Medicine, 5th – 9th June, Salt Lake City, UT, USA. (2010)
 
Mattner, F., Berghofer, P., Shepherd, R., Howell, N., Pham, T., Bourdier, T., Ballantyne, P., Katsifis, A. Ex vivo pharmacological evaluation of the Peripheral Benzodiazepine Receptor radioligand [123I]CLINDE in animal tumour model. 38th Annual Meeting of the Australia and New Zealand Society of Nuclear Medicine, 1st – 6th May, Gold Coast, Australia. (2008)