Bioanalytics

 

Bioanalysis can mean different things to different people. Within ANSTO's human health research infrastructure, we have the capability to measure metabolites and endogenous compounds in biological fluids and analyse biologically active compounds, often using radio-tracers.

 

Bioanalytical techniques can be used to monitor occupational and environmental exposure to chemicals by determining concentrations in biofluids, food or environmental samples, they can be used in forensic toxicology, and in therapeutic drug monitoring. 


Capabilities

Biodistribution in cells

 

  • Light and fluorescent microscopy
  • Deconvolution microscopy 
  • Live cell imaging 
  • Radioreceptor binding (saturation / inhibition / kinetics) 
  • Radiotracer uptake studies 

Membrane biophysics

 

  • Lipid bilayer synthesis and analysis
  • Electrophysiology
  • Impedance spectrometry  

Molecular biology
  • Nucleic acid analysis using real time PCR and high resolution melt analysis 
    Genetic engineering (molecular cloning, transfection, transduction) 
  • Protein expression  
     

Nanodosimetry simulation

  • Computer simulation using Geant4 to model the radiation effect based on the actual biological setup
     

 Physiological functional analysis in cells

 

  • Automated patch clamp of vesicles, organelles and cells 
  • Conventional patch clamp of vesicles and cells
  • Impedance measurements of cells
  • Flow cytometry 
  • Pharmacodynamic assays: cytotoxicity and proliferation 
     

Radiation biology and radioligand biochemistry

 

  • Irradiation of mammalian samples using ionizing (Gamma, X-ray) or non-ionizing radiation (UV)  
  • Compound solubility determination