03/12/18 - Australasian Community for Advanced Organic Semiconductors (AUCAOS) Symposium 2018
Dr Tamim Darwish presented a talk at the Australasian Community for Advanced Organic Semiconductors (AUCAOS) Symposium held in South Australia.
Further information on the symposium can be found here.
02/12/18 - Asian Biophysics Association Symposium
Dr Anthony Duff represented the National Deuteration Facility at the Asian Biophysics Association Symposium in conjunction with the Australian Institute for Biophysics Meeting, held in Melbourne, Victoria.
28/11/18 - 4th Australian Lipid Meeting
Dr Tamim Darwish presented a talk on the National Deuteration Facility at the 4th Australian Lipid Meeting held at the University of Sydney. The talk was entitled "Heavy Lipid Molecules from the National Deuteration Facility for Structure Function Applications".
19/11/18 - ANBUG-AINSE Neutron Scattering Symposium
Professor Peter Holden, Dr Anwen Krause-Heuer and Dr Anthony Duff represented the National Deuteration Facility at the ANBUG-AINSE Neutron Scattering Symposium. Anwen presented a talk entitled 'New contrast options - diversity and specificify of deuteration'
Further details of the meeting can be found here: https://events01.synchrotron.org.au/event/84/
18/11/18 - RACI Medicinal Chemistry and Chemical Biology conference
Dr James Howard presented a poster entitled "Deuterium Labelling in Medicinal Chemistry at the National Deuteration Facility (NDF), ANSTO" at the RACI Medicinal Chemistry and Chemical Biology Conference, held in Brisbane.
Further details of the meeting can be found here: https://www.ivvy.com.au/event/BOD787/
23/10/18 - Successful Funding for ANSTO-University of Wollongong collaboration
Congratulations to Dr Rob Russell and his collaborators from University of Wollongong and Australian Synchrotron who were successful in receiving a seeding fund from ANSTO-University of Wollongong (UoW) Joint Research Projects 2018 scheme. Typically 6 projects per year are awarded.
An application by Dr Ana Heitor (UoW) entitled “Soil stabilization using biopolymer xanthan gum” has received $10,000 in funding. The project is in collaboration with Dr Rob Russell of the National Deuteration Facility and Dr Pimm Vongsvivut ‐ Infrared Microspectroscopy Beamline Scientist at the Australian Synchrotron.
This study aims to investigate the potential use of a biopolymer (xanthan gum) for stabilizing soft soil underlying transport infrastructure (road and rail). The synthesis and deuteration of xanthan gum will be used to study biopolymer/soil particle interaction in the hydrated state.
20/09/18 - Cellulose Dissolution in Ionic Liquid: Ion Binding Revealed by Neutron Scattering
Vikram Singh Raghuwanshi, Yachin Cohen, Guillaume Garnier, Christopher J. Garvey, Robert A. Russell, Tamim Darwish, and Gil Garnier.
Macromolecules 2018, 7649-7655 DOI: 10.1021/acs.macromol.8b01425
Dissolution of cellulose in 1-ethyl-3-methylimidazolium acetate (EMIMAc) ionic liquid (IL) was investigated by small-angle neutron scattering (SANS) with contrast variation. Cellulose and EMIMAc of different deuteration levels provide sufficient contrast in revealing the cellulose dissolution processes.
The deuterated cellulose was produced in the National Deuteration Facility by microbial cells that excrete cellulose nanofibers into their surrounding growth medium. The cells are adapted to growth on D2O (heavy water) and provided deuterated carbon source from which the cellulose is produced.
Deuterated ionic liquid, also from the National Deuteration Facility, was synthesised using a combination of hydrothermal and organic chemistry.
The SANS experiments were performed by Chris Garvey from the Australian Centre for Neutron Scattering (Quokka instrument scientist) and researchers from Monash University and Technion-Israel Institute of Technology.
07/09/18 - Probing liquid crystalline phases for drug delivery applications
Nageshwar R. Yepuri, Andrew J. Clulow, Richard N. Prentice, Elliot P. Gilbert, Adrian Hawley, Shakila B. Rizwan, Ben J. Boyd, Tamim Darwish
Journal of Colloid and Interface Science 2019, 534, 399-407 DOI: 10.1016/j.jcis.2018.09.022
Amphiphilic lipids have the potential to form inverse hexagonal and cubic liquid crystalline phases when they are hydrated by the penetration of organised water channels into the lipid matrix. They have garnered much interest due to their potential to act as biocompatible, environment-responsive delivery vehicles for hydrophobic drugs and membrane-bound peptides/proteins.
Phytantriol is a versatile lipid that forms cubic and hexagonal phases under treatment-relevant conditions and this can be used as a switch to trigger controlled drug release. Phytantriol is also an interfacially-active lipid used by the cosmetics industry that is chemically robust, non-digestible and tends to form particles with bicontinuous cubic phase structures when dispersed with non-ionic surfactants.
To develop effective dosing strategies, phytantriol particles are dispersed in aqueous solution using surfactants to stabilise the large interfacial area formed by internal water channels within the structured lipid particles.
In a recent publication in the Journal of Colloid and Interface Science, researchers from the Monash Institute of Pharmaceutical Sciences, University of Otago and ANSTO, studied deuterated phytantriol-d39 using small angle neutron scattering (SANS) and small angle X-ray scattering (SAXS) to confirm that the dispersed nanoparticles comprising phytantriol-d39 in D2O formed similar liquid crystalline phases as their natural isotopic abundance counterparts.
SANS is an ideal technique for probing the structuring of non-ionic surfactants that stabilise phytantriol-based liquid crystalline particles.
In addition, using deuterated phytantriol, it is possible to obtain excellent contrast between different materials and organic liquid crystalline particles, which enables better resolution of the interfaces within the samples and of the changes that occur.
In this investigation, researchers from the National Deuteration Facility demonstrated the first viable synthesis for the preparation of phytantriol-d39 from phytanic acid. The deuterated version of phytantriol reproduced the liquid crystalline phases observed for hydrogenous phytantriol when dispersed in D2O with Pluronic F127 and Tween 80.
For the first time, the structuring of non-ionic surfactants that stabilise phytantriol-based liquid crystalline particles has been observed by SANS. The different cubic phase structures formed by these two surfactants at room temperature correspond to different organisation of the surfactant stabilisers within cubosomes comprising different intertwining channel structures.
The highly-deuterated phytantriol-d39 described can be used to distinguish or remove signals from phytantriol in nuclear magnetic resonance spectroscopic, mass spectrometric and neutron scattering investigations.
These studies show the strong potential for phytantriol-d39 to play a leading role in the future understanding of the molecular-level interactions of nanostructured particles with blood plasma proteins, exogenous surfactants used as excipients/co-dispersants, components of the digestive milieu and drugs.
25/07/18 - NDF celebrates staff achievements
The National Deuteration Facility held their annual Rewards and Recognition to celebrate staff and team achievements for the 2017/2018 financial year.
These were awarded in four categories, with nominations coming from NDF staff and assessed by an independent panel.
Best Journal Paper: Anwen Krause-Heuer was recognised for her contribution to the Nature Communications article that used deuterated materials to study nanoparticle surface structure.
Commercial/Industry Interaction Colleague: James Howard was recognised for his contributions to a number of commercial projects that generated significant revenue for NDF
Safety and Quality Colleague: Marina Cagnes was celebrated for her continued contributions to the safety of both the chemistry and biology teams of NDF and always striving for continuous improvement.
Delivery and Productivity Colleague: Karyn Wilde was celebrated for her contributions of numerous final molecules for completed proposals within the biology team
15/05/18 - First publication from PELICAN and National Deuteration Facility
The first paper has been published that utilised materials synthesised by the National Deuteration Facility for a study conducted on the cold neutron time-of-flight spectrometer PELICAN at the Australian Centre for Neutron Scattering.
The publication entitled “Measuring the excitations in a new S=1/2 quantum spin chain material with competing interactions” was based on work conducted by Honours student Jack Zanardo from the University of Wollongong under the supervision of Dr Kirrily Rule from the Australian Centre for Neutron Scattering and Associate Professor Michael Lerch from the University of Wollongong.
Inelastic neutron scattering experiments were and used to study the magnetic properties of a spin ½ one dimensional chain, utilizing catena-dichloro(2-chloro-3-methylpyridine)copper(II) to investigate the frustration in a J2/J1 system.
However neutron scattering from hydrogenous material is known to contribute a high background from the large incoherent scattering cross section of H. It was anticipated that in the protonated version of this material (as grown previously) the H would swamp the weak inelastic signal from an S=1/2 Cu2+ ion, therefore a deuterated ligand was used. The deuterated 2-chloro-3-methylpyridine ligand, synthesised by Dr Anwen Krause-Heuer from the Chemical Deuteration team from the National Deuteration Facility.
The interest in this material stems from the relative orientation of the non-magnetic ligand relative to the Cu-chains. This was predicted to induce a range of different J1 interaction and change the inelastic scattering profile.
From the PELICAN data, combined with detailed spin-wave modelling, it appears most likely that the Cu spin-chains formed regions of predominantly one J1 interaction at a time. Thus this system appears to have a random yet equal populations of the differently orientated ligand where chains appear to contain a run of the same orientation. This allows the spinwaves to propagate through the lattice, giving rise to the observed spin excitations. Thus we have been able to confirm that the structural differences did indeed lead to different exchange interactions between neighbouring Cu ions.
It appears most likely that the spin chains formed sections of predominantly one J1interaction at a time. Thus this system appears to have a random yet equal population of the orientation of the ligand where chains appear to contain a run of the same orientation which allows the spinwaves to propagate through the lattice. The structural differences did indeed lead to different exchange interactions between neighbouring Cu ions. It would be very difficult to isolate the structurally different regions to tune particular magnetic properties.
The full publication can be found here.